Protein isoelectric point calculator












Detailed analysis of isoelectric point according different scales for individual proteins

For proteins it is recommend to use "IPC protein", for short peptides (<40 aa) use "IPC peptide". Alternatively use average pI.
For more details "why?" see Results section.

Isoelectric point of protein is: 5.98


                           ●                                         average pI (pI=5.571)
                             ●                                       IPC protein (pI=5.982)
                             ●                                       IPC peptide (pI=5.974)
                             ●                                       Toseland (pI=5.95)
                            ●                                        Thurlkill (pI=5.834)
                           ●                                         Nozaki_Tan (pI=5.65)
                           ●                                         Dawson (pI=5.699)
                           ●                                         DTASelect  (pI=5.55)
                             ●                                       EMBOSS     (pI=6.099)
                          ●                                          Grimsley   (pI=5.499)
                                      ●                              Patrickios (pI=7.7)
                           ●                                         Rodwell    (pI=5.55)
                           ●                                         Sillero    (pI=5.699)
                             ●                                       Solomon    (pI=5.999)
                             ●                                       Lehninger  (pI=6.015)
                             ●                                       Wikipedia  (pI=5.925)
●                                                                     ProMoST    (pI=0.001)
————◊————◊————◊————◊————◊————◊————◊————◊————◊————◊————◊————◊————◊————>
    1    2    3    4    5    6    7    8    9    10   11   12   13  
* average pI includes all scales except Patrickios (highly simplified scale frequently leading to bizzare results)
For more details about pKa used for calculation according different scales see a table
Input sequence:

>unnamed


Charge at pH=5.5 (lysosome): 0    Charge at pH=7.4 (cytoplasm): -0    Charge at pH=8.0 (mitochondria): -0.1
Your protein (peptide) has 0 amino acids. 
Ala
0
 
Phe
0
 
Val
0
 
Cys
0
 
Ser
0
 
Asp
0
 
Lys
0
 
Met
0
 
Gly
0
 
Trp
0
 
Asn
0
 
Thr
0
 
Glu
0
 
Arg
0
 
Pro
0
 
Ile
0
 
Leu
0
 
Gln
0
 
Tyr
0
 
Sec
0
 
His
0
Protein mass: 18.01524 Da

Download results in CSV format


Please, support the project and cite: Kozlowski LP (2016) IPC - Isoelectric Point Calculator. Biology Direct 11:55.


You may also be interested to check this database: Proteome-pI - Proteome Isoelectric Point Database - predicted isoelectric point for ~21 million proteins across 5,029 organisms

Date: 04:04, 18th April 2024

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Contact: Lukasz P. Kozlowski